Entrepreneurial characteristics are among the characteristics individuals should have in our century. Although there is an awareness of the necessity of gaining entrepreneurial characteristics at all levels of education, unfortunately, the number of studies focusing on entrepreneurship education at the preschool level is limited. For this reason, this chapter focuses on the promotion of entrepreneurship in preschool science education and it aims to draw attention to entrepreneurship education at the preschool level, the use of science activities in preschool entrepreneurship education, and guide researchers who want to work in this field and the preschool teachers who want to give entrepreneurship education. For this purpose, in this chapter, a basic framework was established for entrepreneurship education at the preschool level, and the role of science activities in preschool entrepreneurship education and the development of effective entrepreneurial preschool science activities was expressed through a non-systematic literature review. In addition, three different practical entrepreneurial preschool science activities developed by the author were introduced and suggestions were made to apply them in preschool classes.